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G3BP1 antibody#38645

G3BP1 antibody
G3BP1 antibody
G3BP1 antibody
G3BP1 antibody
G3BP1 antibody
G3BP1 antibody
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产品总价:
产品详情

产品名称G3BP1 antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by affinity purification using immunogen.

应用WB,IHC,IF

种属反应性Human,Mouse,Rat

特异性The antibody detects endogenous level of total G3BP1 protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant protein of human G3BP1.

基因/蛋白名称G3BP1

别名G3BP; HDH-VIII;

数据库入口号Swiss-Prot#: Q13283
NCBI Gene ID: 10146

UniprotQ13283

实际分子量52kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500 - 1:2000
Immunofluorescence: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using G3BP1 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat liver using G3BP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using G3BP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using G3BP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using G3BP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using G3BP1 antibody at dilution of 1:100 (40x lens).
背景
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

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et al,Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling In Nucleic Acids Res On2023 Jul 7byNevraj S Kejiou , Lena Ilan et al..PMID:37224531, , (2023),
PMID: 37224531
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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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